Debasish Mohapatra

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Dr. Debasish Mohapatra has completed his Ph.D. in Biotechnology from the Department of Botany, Ravenshaw University. He is currently serving as Guest Faculty in the Department and teaches Botany and Biotechnology courses. His doctoral work was on the bioremediation of organophosphate insecticide (malathion and parathion) using Aspergillus niger. He has 9 research papers in national and international journals. He has also applied for one patent which is the outcome of his doctoral research. He has participated and presented his work in more than 12 conferences/ seminars / symposium. He also attended a workshop on ‘Skill and Entrepreneurship Development Program’ funded by DST, Govt. of Odisha. He was selected and successfully completed BCIL (Biotech Industrial Training Program) 2012 organised by Department of Biotechnology (DBT), Government of India, New Delhi. Dr. Mohapatra was awarded “Prof. B. P. Choudhury Memorial Award” for Best Oral Presentation by Odisha Botanical Society (OBS) in 2015 at the 40th Annual Conference of OBS organized by Fakir Mohan University, Balasore. He also got the Second Prize in Poster Presentation (Session: Applied Microbiology) during the National Conference on Emerging Trends in Plant Science Research (ETPSR-2020) held at Ravenshaw University. He is also pursuing his post-doctoral research in the field of Environmental Biotechnology and Plant Biotechnology at Ravenshaw University.

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Bioremediation is a natural process for the decontamination of soil and groundwater with organisms. It involves waste management techniques to effectively and ecologically remove pollutants from the environment. Different living organisms, plants and fungi, and bacteria also known as bioremediators can remove or detoxify these hazardous wastes. Biotechnological exploitation and management of contaminated soil and water in situ and ex situ by using such fungi is considered to be mycoremediation. The use of native and/or alien microflora enhances soil health and also help in bioremediation of the agroecosystem of soil. Different species of Aspergillus have shown their efficiency to degrade OP chemicals. A malathion tolerant Aspergillus niger MRU01, developed by prolonged malathion exposure, was tested for its efficiency to remove malathion as well as three other commonly used OP insecticides, viz., parathion, chlorpyrifos and dimethoate. The tolerant strain grew efficiently in the presence of 500 µM of malathion and the biomass production of A. niger M-RU01 was significantly higher than of the untreated as well as treated wild type (A. niger MTCC16404). The activities of phosphatases and esterase of A. niger M-RU01 strain was significantly higher than of the wild type when treated with malathion, parathion, chlorpyrifos, and dimethoate. The tolerant strain was able to remove all the insecticides more efficiently than the wild type. The strain can be used for field application to metabolically remove diverse OP compounds.

The tolerant strain was mass cultured and the mycelium with its spore were isolated from the culture and mixed with suitable carried for the development of the fungal preparation. The efficiency of the preparation was tested under different oxygen realm and moisture condition to obtain the ambient conditions for storage. The fungal preparation was highly effective in removing malathion as well as the other three OP insecticides. A storage period of 45 days may be considered ideal for maintaining the preparation in the active state, and is recommended for field application after evaluating its efficacy under field conditions.

  1. Mohapatra D, Rath S.K. and Mohapatra P.K. (2021). Evaluating a preparation of malathiontolerant Aspergillus niger MRU01 for accelerated removal of four organophosphorus insecticides. Journal of Chemical Technology and Biotechnology. 96(6). 1603-1610. https://doi.org/10.1002/jctb.6679.
  2. Shasmita, Mohapatra D, Mohapatra PK, Naik SK, Mukherjee AK (2019) Priming with salicylic acid induces defense against bacterial blight disease by modulating rice plant photosystem II and antioxidant enzymes activity. Physiological and Molecular Plant Pathology. 108 (2019). 101427.  https://doi.org/10.1016/j.pmpp.2019.101427. 
  3. Mohapatra D, Rath S.K. and Mohapatra P.K. (2018). The growth and biochemical responses of Aspergillus niger to treatment of malathion. Plant Science Research 40(1&2). 01-06. ISSN 0972-8546. 
  4. Mohapatra D, Rath S.K and Mohapatra P.K (2018). Bioremediation of insecticide by white rot fungus and its environmental relevance. In: Prasad R (eds) Mycoremediation and Environmental Sustainability. Fungal Biology. Springer, Cham pp. 181-212.
  5. Mohapatra D, Singh N.R. and Rath S.K. (2018) Prospects and application of Azospirillum spp. as a natural agricultural biofertilizer. In: Kumar P, Patra JK and Chandra P (eds) Advances in Microbial Biotechnology. CRC Press (Taylor and Francis Group), pp. 153-174.
  6. Mohapatra D, Singh N.R. and Rath S.K. (2017) Recent Advances in Natural Product from Macro Fungus: A Territory Unexplored. In: Panda SK (eds) Recent Advances in Natural Products. Studium Press Llc. USA. pp. 501-522.
  7. Mohapatra D, Singh N.R. and Rath S.K. (2017) Anti-Glycation Agents/Products from Natural Sources: A Path Unexplored. In:   Patra JK, Ratha SK and Jayabalan S (eds) Natural Products in Food Prospects and Application. Studium Press Llc. USA. pp. 383-400.
  8. Mohapatra D, Rath S.K. and Mohapatra P.K. (2016). Biodegradation of insecticides and herbicides by Aspergillus species: A review. Plant Science Research 38(1&2). 23-29. ISSN 0972-8546.
  9. Mohapatra D, Singh N.R. and Rath S.K. (2015) Methods for Identification and Conservation of Macro Fungus: A Probable Global Food. International Journal of Biological Sciences and Engineering. 6(1): 16-23.

Teaching :

  1. Genetics
  2. Biochemistry
  3. Immunology
  4. Instrumentation and Computational Biology
  5. Microbiology
  6. Molecular Biology
  7. Genetic Engineering
  8. Environmental Biotechnology
  9. Plant Biotechnology