Molecular Biology and Biotechnology

Khirod Kumar Sahoo

Room: 301-A,
New Academic Block

+91-6370903711

khirod555[at]gmail[dot]com

Dr. Khirod K. Sahoo is Assistant Professor in the Department of Botany since 2014. He has 10 years of research experience. He has more than 15 publications in peer reviewed International journals and 01 national patent to his credit. He did his doctoral research on developing the salinity and drought tolerant transgenic rice through pyramiding genes in 2010 at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi and in collaboration with School of Life Sciences, JNU, New Delhi. He further did his postdoctoral research for more than 2 years at ICGEB, New Delhi on transgenic rice and on RNAi Technology. After that, he served as Senior Technical Officer in Biotechnology Division at CSIR-Institute of Himalayan Bioresource Technology (IHBT), Govt. of India, Palampur. Currently, he has research collaboration with ICAR-National Rice Research Institute (NRRI), Cuttack and Imgenex India, Pvt. Ltd., Bhubaneswar. Currently his research is focused on crop improvements through plant genetic engineering, plant proteomics and metabolomic study. His research aims to make plants economically viable through tissue culture and hydroponic methods.

Selected Publications

  1. Sahoo B, Nayak I, Parameswaran C, Kesawat MS, Sahoo KK, Subudhi HN, Balasubramaniasai C, Prabhukarthikeyan SR, Katara JL, Dash SK, Chung SM. (2023) A Comprehensive Genome-Wide Investigation of the Cytochrome 71 (OsCYP71) Gene Family: Revealing the Impact of Promoter and Gene Variants (Ser33Leu) of OsCYP71P6 on Yield-Related Traits in Indica Rice (Oryza sativa L.). 12(17):3035. ISSN- 2223-7747 (Impact Factor- 4.658).
  2. Muduli N, Aparna S, Patri M, Sahoo KK*. (2023) Saffron stigma extract and crocin play an important neuroprotective role in therapeutic measures against benzo [a] pyrene-induced behavioral alterations in zebrafish. Drug and Chemical Toxicology. 30:1-2. ISSN- 1525-6014 (Impact Factor- 2.597).
  3. Ojha P, Kar NP, Nayak S, Patra AK, Sahoo KK* (2021) Isolation of a broad spectrum antimicrobial producing thermophilic Bacillus and characterization of its antimicrobial protein. Archives of Microbiology. DOI 10.1007/s00203-020-02162-w. ISSN- 0302-8933. (Impact Factor- 2.667).
  4. Sahoo KK, Gupta BK, Kaur C, Joshi R, Pareek A, Sopory SK, Singla-Pareek SL (2021) Methylglyoxal-glyoxalase system as a possible selection module for raising marker-safe plants in rice. Physiology and Molecular Biology of Plants. 27:2579–2588 (2021). https://doi.org/10.1007/s12298-021-01072-5. ISSN- 09740430, 09715894. (Impact Factor- 3.023).
  5. Joshi R#, Sahoo KK#, Singh AK, Anwar K, Pundir P, Gautam RK, Krishnamurthy SL, Sopory SK, Pareek A, Singla-Pareek SL. (2020) Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions. Journal of Experimental Botany, 71, (2) 653–668. ISSN 0022-0957 (#Equally First Author) ISSN- 653 -668. (Impact Factor- 7.378).
  6. #Gupta, B.K., #Sahoo, K.K., Ghosh, A., Tripathi, A.K., Anwar, K., Das, P., Singh, A.K., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. (2018) Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice. Plant Cell Environment. 41:1186–1200 (#Equally First Author). ISSN- 1186-1200. (Impact Factor- 7.947).
  • Group
  • Research
  • Publications
  • Teaching

Nupur Naik

Ph.D. awarded in 2018

Ravindra Donde Dattu

Ph.D. awarded in 2020

Purusottam Ojha

Research Scholar

Namita Muduli

Research Scholar

M.Phil.

  1. Bhagyashree Dash in 2019
  2. Debasish Tarai in 2018
  3. Rajalaxmi Sahoo in 2017
  4. Sashmita Nayak in 2016

Plant Molecular Biology and Biotechnology lab

The Plant Molecular Biology and Biotechnology lab is supported by Science and Engineering Research Board (SERB), govt. of India and Science and Technology Department, govt. of Odisha. We have expertise in the genomics, proteomics, metabolomics and genetic engineering including Agrobacterium mediated plant (Rice, Tobacco and Saffron) transformation with fundamental knowledge of plant tissue culture. We also characterize several plant metabolites and understand its effect on anxiety and behavioral response using Zebra fish model.

Isolation and characterization of a broad spectrum antimicrobial producing thermophilic Bacillus

The thermophilic bacterium having antimicrobial activity was isolated and identified as Bacillus paralicheniformis by morphology, microscopy, and 16S-rRNA. The secreted antimicrobial was a 16.74 kDa protein homologous of chicken-lysozyme-C. The discovered lysozyme has the extraordinary potential to lyse Gram-positive bacteria, yeast, fungus, and more efficiently lyse chick-lysozyme-C resistant lipopolysaccharide rich Gram negative bacteria’s outer cell wall (Ojha et al. 2020).

Crop improvement and signal transduction

We are doing research on crop improvements through understanding the gene regulation in the complex mechanism of stress and signaling, applying the basic molecular biology tool, understanding genetic diversity and to use the information to develop high crop yield using new technology. miRNA involved in drought and salinity stress response, including ABA response, auxin signaling, osmoprotection and antioxidant defense by down-regulating the response target gene.

Plant tissue Culture: We are working to establish protocols for micropropagation of economically important plants which possesses high commercial values. 

Hydroponic culture: In addition, we are also working on mass scale establishment of the economically important plants under hydroponic soilless condition.

Mushroom culture: We are trying to develop an efficient mushroom cultivation technology for better quality and quantity of mushroom production.

Research Grants: name and sponsoring agent

  1. Science and Engineering Research Board (SERB), Govt. of India funded EMR project –   Completed 
  2. Science and Technology Department, Govt. of Odisha funded research project – Ongoing

Research Article Publications

  1. Ojha P, Kar NP, Nayak S, Patra AK, Sahoo KK (2020) Isolation of a broad spectrum antimicrobial producing thermophilic Bacillus and characterization of its antimicrobial protein. Archives of Microbiology. DOI 10.1007/s00203-020-02162-w. (Impact Factor- 1.835).
  2. Donde R, Mohapatra S, Baksh SKY, Padhy B, Mukherjee M, Roy S, Chattopadhyay K, Anandan A, Swain P, Sahoo KK, Singh ON, Behera L, Dash SK (2020) Identification of QTLs for high grain yield and component traits in new plant types of rice. PLoS One. 15(7): e0227785. (Impact Factor- 2.740).
  3. Joshi R, Sahoo KK, Singh AK, Anwar K, Pundir P, Gautam RK, Krishnamurthy SL, Sopory SK, Pareek A, Singla-Pareek SL. (2020) Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions. Journal of Experimental Botany, 71, (2) 653–668. ISSN 0022-0957 (#Equally First Authour) (Impact Factor- 5.908).
  4. Donde Ravindra, Kumar Jitendra, Gauda Gayatri, Gupta Manoj Kumar, Mukherjee Mitadru, S.K.Y. Baksh1, Mahadani P., Sahoo K.K., Behera L., Dash S. K. (2019) Assessment of genetic diversity of drought tolerant and susceptible rice genotypes using microsatellite markers. Rice Science, 26(4): 239-247 ISSN 1672-6308 (Impact Factor- 1.521).
  5. Donde Ravindra, Gupta Manoj Kumar, Gouda Gayatri, Kumar Jitendra, Vadde Ramakrishna,  Sahoo  K.K, Dash Sushanta Kumar, Behera Lambodar (2019) Computational characterization of structural and functional roles of DREB1A, DREB1B and DREB1C in enhancing cold tolerance in rice plant. Amino Acid. 51, 839–853, ISSN: 0939-4451 (Print) 1438-2199 (Online) (Impact Factor- 2.906).
  6. Joshi, R., Sahoo, K.K., Tripathi, A.K., Kumar, R., Gupta, B.K., Pareek, A., Singla-Pareek, S.L. (2018) Knockdown of an inflorescence meristem-specific cytokinin oxidase – OsCKX2 in rice reduces yield penalty under salinity stress condition. Plant Cell Environment. 41:936–946 (Impact Factor- 6.362).
  7. Gupta, B.K., Sahoo, K.K., Ghosh, A., Tripathi, A.K., Anwar, K., Das, P., Singh, A.K., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. (2018) Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice. Plant Cell Environment. 41:1186–1200 (#Equally First Author) (Impact Factor- 6.362).
  8. Naik N, Rout P, Umakanta N, Verma RL, Katara JL, Sahoo KK, Singh ON, Samantaray S (2017) Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture. Plant Cell Tissue and Organ Culture. 128:691. (Impact Factor- 2.196).
  9. Naik N, Rout P,  Verma RL, Umakanta N, Katara JL, Sahoo KK, Singh ON, Samantaray S (2017) Development of promising doubled haploids from indica rice hybrid: evaluation of  uniformity and stability through morphological and molecular markers. International Journal of Agricultural Sciences 47 (8): 1987-1992. 
  10. Pradhan A, Sahoo KK (2015) Role of RNAi in Salinity and Drought Tolerance in Plants: Special Reference to Rice. Special issue on Rice. American Journal of Plant Science. 6:1990-2008. 
  11. Kumar R, Mustafiz A, Sahoo KK, Sharma V, Samanta S, Sopory SK, Pareek A and Singla-Pareek SL. (2012) Functional screening of cDNA library from a salt tolerant rice genotype Pokkali identifies Mannose -1-phosphate guanyl transferase gene (OsMPG1) as a key member of salinity stress response. Plant Molecular Biology. (DOI 10.1007/s11103-012-9928-8) (Impact Factor- 3.3).
  12. Sahoo KK, Tripathi AK, Pareek A, Sopory SK and Singla-Pareek SL. (2011) An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars. Plant Methods. (doi:10.1186/1746-4811-7-49). (Impact Factor- 3.5).

Book Chapters Publications

  1. Dash SK, Sahoo KK, Chattopadhyay K, Behera L, Pradhan SK and Mohapatra SD (2016) High Beta Carotene Rice in Asia: Techniques and Implications. Springer. (Equal First author) page- 359-374.
  2. Sahoo KK, Mallik J, Pareek A, Singla-Pareek SL and Sopory SK (2011) Tackling abiotic stresses through gene pyramiding: strategies and applications. Genomics and Crop In Improvement: Relevance and Reservations pg 330-337, Hyderabad, India.
  3. Mustafiz A, Sahoo KK, Singla-Pareek SL and Sopory SK (2010) Metabolic engineering of glyoxalase pathway for enhancing stress tolerance in plants. In Methods in Molecular Biology, Plant Stress Tolerance. Ed Ramanjulu Sunkar, Humana Press, USA. ISSN: 1064-3745, 2010, 95-118.
  4. Sahoo KK, Tripathi AK, Pareek A and Singla-Pareek SL (2013) Taming drought stress in rice through genetic engineering of transcription factors and protein kinases. Global Science Book. Plant stress, special issue1:60-72.

Patent

  1. An improved methodology for high frequency of regeneration of genetically transformed calli of an indica rice – Oryza sativa L. cv IR64 Inventors: Singla-Pareek SL, Sahoo KK and Sopory SK (2008) International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, Pin- 110067, India.  Indian Patent Application: 1736/DEL/2008.

Teaching :

  1. Recombinant DNA Technology
  2. Plant Biotechnology
  3. Molecular Biology
  4. Genetics
  5. Plant Tissue culture
  6. Instrumentation
  7. Molecular marker assisted selection
  8. Intellectual Property Rights and ethics