Plant Biochemistry & Bioinformatics

Nihar Ranjan Singh

Room No.

+91-9439938581

nihar.singh@gmail.com

Dr. Nihar Ranjan Singh, completed his M.Sc. in Botany from erstwhile Ravenshaw College, with a specialization in Biochemistry. He also had a Post-Graduation Diploma in Bioinformatics from the Institute of Bioinformatics and Applied Biotechnology, Bangalore. He completed his doctoral studies from Université de la Réunion, France (2005-2008) and his doctoral research was on glycoxidative stress and protein carbonylation in adipocytes. After completing his doctoral work, he pursued postdoctoral research at CMRG, School of Physiological Sciences, Stellenbosch University, RSA (2009-2010) before joining Ravenshaw University as an assistant professor. He has more than 11 years of teaching and research experience in the field of plant biology, biochemistry, molecular biology, and bioinformatics. His present research is on  different aspects of plant biochemical processes like proteome modification using multi-omics approaches. His interest areas include traditional knowledge of medicinal plants and anti-glycation properties of ethnomedicinal plants of Odisha. He has published more than 30 scientific articles in peer-reviewed journals of national and international repute. Apart from that he has two books and five book chapters to his credit. He is also the course coordinator of the newly established  Department of Biochemistry.

Selected Publications

  1. Pradhan, S. K., Singh, N. R., Dehury, B., Panda, D., Modi, M. K., & Thatoi, H. (2019). Insights into the mode of flavin mononucleotide binding and catalytic mechanism of bacterial chromate reductases: A molecular dynamics simulation study. Journal of cellular biochemistry, 120(10), 16990-17005.
  2. Swain, C. K., Bhattacharyya, P., Nayak, A. K., Singh, N. R., Neogi, S., Chatterjee, D., & Pathak, H. (2018). Dynamics of net ecosystem methane exchanges on temporal scale in tropical lowland rice. Atmospheric environment, 191, 291-301.
  3. Pradhan, S. K., Singh, N. R., Rath, B. P., & Thatoi, H. (2016). Bacterial chromate reduction: a review of important genomic, proteomic, and bioinformatic analysis. Critical Reviews in Environmental Science and Technology, 46(21-22), 1659-1703.
  4. Roche, M., Rondeau, P., Singh, N. R., Tarnus, E., & Bourdon, E. (2008). The antioxidant properties of serum albumin. FEBS letters, 582(13), 1783-1787.
  5. Rondeau, P., Singh, N. R., Caillens, H., Tallet, F., & Bourdon, E. (2008). Oxidative stresses induced by glycoxidized human or bovine serum albumin on human monocytes. Free Radical Biology and Medicine, 45(6), 799-812.
  • Group
  • Research
  • Publications
  • Teaching

Ms. Upasana Sahoo

Research Scholar

Mr. Raj Kishore Sahoo

Research Scholar

Ms. Anindita Mishra

Research Scholar

Mr. Jitendra Kumar

Research Scholar

Mrs. Sai Aparna

Research Scholar

Mr. Sujit Kumar Das

Research Scholar

Mr. Sukanta Kumar Pradhan

Ph.D. awarded in 2020

Mr. Shakti Prakash Mohanty

Ph.D. awarded in 2020

Mr. Chinmaya Kumar Swain

Ph.D. awarded in 2019

Mrs. Ratnalipi Mohanty

Ph.D. awarded in 2018

M.Phil.

  1. Ms. Banani Barai (2011)
  2. Ms. Omprava Das (2012)
  3. Mr. Pramod Kumar Tripathy (2013)
  4. Ms. Sanjana Singh (2014)
  5. Ms. Sarita Jayanti Goswami (2016)
  6. Ms. Sucharita Satapathy (2018)
  7. Ms. Anindita Mishra (2019)
  8. Ms. Maheswari Behera (Ongoing)

Research

EVOLUTIONARY ANALYSIS OF CRY TOXINS:

Structural and functional analysis of Cry proteins from different insect group is performed to understand the functional domain arrangements at sequence level as well as at structural level involving both experimental and predicted 3-dimensional models. The analysis of evolutionary relationship involving all documented Cry proteins at the sequence, domain, and structural levels is done using different bioinformatics tools. Evolutionary analysis revealed that some Cry proteins having toxicity for a specific insect order are found to be clustered for another different insect order. Three dimensional (3D) structure analysis of both experimental and predicted models revealed that proteins might have toxicity for a specific insect order differ in their structural arrangements and was observed in Cry proteins belonging to different insect orders. (https://rdcu.be/ckQBh)

 

BIOCHEMICAL PATHWAY OF ELICITORS:

Elicitors can act through various pathways including ROS and alter various biochemical and physiological processes inside the cell. Although elicitor-mediated responses initiate at the membrane, it can have a series of responses to multiple cellular targets through multiple channels or pathways. Such an elaborative and extensive consequence of elicitor-mediated responses are not yet fully understood, however, recent developments in this field of plant biotechnology and secondary metabolite production put forward new insights into the mechanism of elicitor action and its application. A model depicting the sequence of events following recognition and binding of an elicitor to receptor in plant cells is demonstrated. (DOI: https://doi.org/10.1007/978-981-10-8666-3_9)

 

ETHNOBOTANICAL SURVEY OF PARASITIC PLANTS

Parasitic plants are specialized plant which depends on host plant without contributing to their benefit and sometimes causing extreme damage to the host. Occurrence of several species of such parasitic plants in Odisha is a wealthy acquaintance for us. In this collective work, we surveyed and compiled parasitic plants from different parts of the state of Odisha. (ISBN: 978-81-935702-3-4)

 

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  1. Das, S.K., Pradhan, S.K., Samal, K.C., Singh, N. R. (2021) “Structural, functional, and evolutionary analysis of Cry toxins of Bacillus thuringiensis: an in silico study”. Egyptian Journal of Biological Pest Control 31, 44. 
  2. Pradhan, S.K., Singh, N. R., Kumar, U., Mishra, R.S., Perumal, R.C., Benny, J., Thatoi, H. (2020) Illumina MiSeq based assessment of bacterial community structure and diversity along the heavy metal concentration gradient in Sukinda chromite mine area soils, India” Ecological Genetics and Genomics, 15, p.100054. 
  3. Pradhan, S. K., Singh, N. R., Dehury, B., Panda, D., Modi, M. K., & Thatoi, H. (2019). Insights into the mode of flavin mononucleotide binding and catalytic mechanism of bacterial chromate reductases: a molecular dynamics simulation studies” Journal of Cellular Biochemistry, 120(10), 16990-17005.
  4. Swain, C. K., Nayak, A.K., Bhattacharyya, P., Chatterjee, D., Chatterjee, S., Tripathi, R., Singh, N.R., and Dhal, B (2018) Greenhouse gas emissions and energy exchange in wet and dry season rice: eddy covariance-based approach” Environmental Monitoring and Assessment, 190:423, 1-17.
  5. Swain, C. K., Bhattacharyya, P., Nayak, A. K., Singh, N. R., Neogi, S., Chatterjee, D., & Pathak, H. (2018) Dynamics of net ecosystem methane exchanges on temporal scale in tropical lowland rice” Atmospheric Environment, 191, 291-301.
  6. Kumar, S., Mahanti, P., Singh, N. R., Rath, S. K., Jena, P. K., Patra, J. K. (2017). Antioxidant activity, antibacterial potential and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India. Brazilian Journal of Pharmaceutical Sciences, 53(4).
  7. Pradhan, S.K., Singh, N.R., Rath, B.P., Thatoi, H (2016) Bacterial chromate reduction: A review of important genomic, proteomic, and bioinformatic analysis”, Critical Reviews in Environmental Science and Technology, vol. 0, no. 0, 1–45.
  8. Swain, C.K., Bhattacharyya, P., Singh, N.R., Neogi, S., Sahoo, R.K., Nayak, A.K., Zhang, G., and Leclerc, M.Y. (2016) Net ecosystem methane and carbon dioxide exchange in relation to heat and carbon balance in lowland tropical rice” Ecological Engineering, 95, page 364–374.
  9. Mohanty, R., Das, S. K., Singh, N. R.,& Patri, M. (2016). Withania somnifera leaf extract ameliorates benzo [a] pyrene-induced behavioral and neuromorphological alterations by improving brain antioxidant status in zebrafish (Danio rerio). Zebrafish, 13(3), 188-196.
  10. Murmu, P., Kumar, S., Patra, J. K., Singh, N.R., Rath, S.K. (2016) Ethnobotanical, Nutritional, Phytochemical and Antimicrobial Studies of Garcinia xanthochymus Fruit Extracts” BBJ, 13(2): 1-11.
  11. Mohapatra, P.K., Singh, N.R. (2014) Teaching the Z-Scheme of Electron Transport in Photosynthesis by Govindjee” Photosynthesis Research, Volume 123, Issue 1, pp 105–114.
  12. Singh, N.R., Rondeau, P., Bourdon, E. (2009) Identification of up-regulated low molecular weight proteins in human adipocytes treated with glycoxidized albumin” The Open Obesity Journal, 2009, 2, 00-00
  13. Rondeau, P., Singh, N.R., Caillens, H., Bourdon, E. (2008) Oxidative stresses induced by glycated human or bovine serum albumin on Human monocytes” Free Radical Biology and Medicine 45:799-812.
  14. Roche, M., Rondeau, P., Singh, N.R., Tarnus, E., Bourdon, E. (2008) The antioxidant properties of serum albumin” FEBS Lett. 2008 Jun 11; 582(13):1783-7.
  15. Singh, N.R., Rondeau, P., Hoareau, L., Bourdon, E. (2007) Identification of preferential protein targets for carbonylation in human mature adipocytes treated with glycated albumin” Free Radical Research, 41(10):1078-88.

Book Chapters

  1. Mohapatra, D., Singh, N.R., Rath, S.K (2017) Anti-Glycation Agents/Products from Natural Sources: A Path Unexplored” Natural Products in Food : Prospects and Applications, Studium Press LLC, USA.
  2. Mohapatra, D., Singh, N.R., Rath, S.K (2017) Recent Advances in Natural Product from Macro Fungus: A Territory Unexplored” Recent Advances in Natural Products: Studium Press LLC, USA.
  3. Shasmita, Singh, N.R., Rath, S.K., Behera, S.K., Naik, S.K. (2017) In vitro Secondary Metabolite Production Through Fungal Elicitation: An Approach for Sustainability” Chapter 9, Springer: Fungal Nanobionics – Principles and Applications.
  4. Sahoo, B.C., Rath, S.K., Singh, N.R. (2018) Comparative Assessment of Protein Extraction Methods for Leaf and Seed of Vigna radiata (L.) R.Wilczek Plant” Narosa Publishers.

Book Published

  1. Sahu, S.C., Kumar, S., Singh, N.R. (2018) Parasitic Plants of Odisha” Published by Ambika Prasad Research Foundation. ISBN: 978-81-935702-3-4 .
  2. Kumar, S., Biswal, S.K., Mishra, S., Singh, N.R., Balakrishnan, P., Kumawat, N.K., Dhal, N.K. (2019) Medico-Biowealth of Odisha. Published by Ambika Prasad Research Foundation. ISBN: 978-81-938861-4-4.

Teaching :

  1. Plant Biochemistry
  2. Molecular Biology
  3. Bioinformatics