Biophysical Research Group

Bairagi C. Mallick

Room No. - 103B,
Academy Block - 1

+91-9178890581

bcmallick[at]ravenshawuniversity [dot] ac [dot] in

Dr. Mallick holds the post of Associate Professor in the Department of Chemistry. He completed his Ph.D. in 2007 at IIT Bombay, India with Prof. Nand Kishore. Then, he moved to Seoul National University (SNU), Republic of Korea and worked with Prof. Sa-Ouk Kang as a KB-21 postdoctoral researcher. He joined the Department of Chemistry, Ravenshaw faculty in 2010. He has 11 years of teaching and 14 years of post Ph.D. research experience. He has published in international peer review journals and written edited book chapters. He was awarded the Raman Postdoctoral Fellowship in 2016 by UGC, Govt. of India to conduct advance research in the University of Iowa, USA. He is a trained biophysical chemist with expertise in protein structure and functional nanoparticles research.

His research group is interested to unravel the complexity of proteins involved in antibiotics resistance, and the nano-bio interface chemistry of biogenic/engineered nanoparticles to facilitate their biomedical applications

Selected Publications

  1. Pattanayak, B. S.; Priyadarshinee, M.; Dehury, B.; Jha, S.; Beuria, T. K.; Soren, D.; Mallick, B. C. Kanamycin mediated conformational dynamics of Escherichia coliouter membrane protein TolC. Frontiers in Molecular Biosciences 2021, 8, 636286. doi.org/10.3389/fmolb.2021.636286
  2. Behera, N.; Arakha,M.; Soren, S.; Pattanayak, B. S.; Priyadashsinee, M.; Jha, M.; Mallick, B. C. Oxidative stress generated at nickel oxide nanoparticle interface results in bacterial membrane damage leading to cell death. RSC Advances 20199, 24888-24894.  doi.org/10.1039/C9RA02082A
  3. Nayak, P. S.; Arakhaa, M.; Kumar, A.; Asthana,S.; Mallick, B.C.; Jha, S. Optimization of online silver nanoparticle biofabrication using Bacillus thuringiensisRSC Advances 2016, 6, 8232-8242. org/10.1039/C5RA21281B
  4. Arakhaa, M.; Pala, S.; Samantarraia, D.; Panigrahid, T.K.; Mallick, B. C.; Pramanikb, K.; Mallick, B.; Jha, S. Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interfaces. Scientific Reports 2015, 5:14813. doi:10.1038/srep14813.
  5. Arakha, M.; Saleem, Md.; Mallick, B. C.; Jha, S. The effects of interfacial potential on antimicrobial propensity of ZnO nanoparticle, Scientific Reports 2015, 5: 9578. doi:10.1038/srep09578.
  • Group
  • Research
  • Publications
  • Teaching

Prangya P. Panda

Research Scholar

Mamali Priyadarshinee

Research Scholar

Nibedita Behera

Research Scholar

Chitranjali Jena

Research Scholar

chitranjaliijena95@gmail.com

Sulata Kumari Sahu

Research Scholar

WhatsApp Image 2022-01-06 at 1.53.25 PM

Manaswini Patra

Research Scholar

Lab Alumni

Dr. Prakasini Satapathy

Molecular characterisation of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis cases of Odisha

Dr. Biraja S. Pattanayak

Biochemical and Biophysical Characterization of Drug Efflux Protein TolC in Escherichia coli.

Dr. Sanjukta Badhai

Effective Delivery of Phytosterol Based Drugs Against Colon Cancer

The treatment of microbial infection and cancer has suffered greatly in the present century of pathogenic dominance. This prevailing situation has become a challenge to the active medical partitioners and to the scientific community. Thus, our long-term goal is to understand the mode/mechanism of antibiotics resistance both in bacteria/cancer  cells and identify naturally available drugs/antibiotics or develop biogenic/engineered nanoparticles to support/modify or replace the conventional used antibiotics that has led to cause resistance.

The emergence/existence of antibiotic resistance bacteria has evidenced to be associated with the overexpression of efflux pumps. These efflux pumps are either membrane or membrane associated proteins that have mediated antibiotic resistance through efflux mechanism. Thus, understanding the mechanism of antibiotics binding of these proteins and their efflux mechanism through the membrane is a real challenge, but in long run, it will help to improve the present condition of antibiotic resistance. Our approached is to purify membrane proteins in active state, and explore the antibiotics binding dynamics through biophysical techniques and substernal it through bioinformatic analysis.


The protective effects of naturally present phytosterols have been studied in various models and approaches. However, the exact mechanism of phytosterol action is still unknown. In our approach, we have identified different sources for extraction and purification of phytosterol of  interest and test their efficacy as an anticancer agent against breast cancer cells.

The rapid growth in nanotechnology has become the likelihood of engineered nanomaterials that coming into environmental and human contact. Nanoparticles interacting with cells membranes, DNA, proteins and organelles establish a series of nano-bio interfaces.  The study in our group aimed to evaluate the antimicrobial propensity of biogenic/synthesized NPs with significant anti-microbial activity. This will established potential use of NPs in biomedical and pharmaceutical sciences over the conventional antibiotics

Projects :

1. Interaction Role of PKM2 and HIF-1α in Breast Cancer Progression
[SERB-DST, Govt. of India]

2. Biochemical and Biophysical Characterization of Efflux Protein-Mediated Drug
Resistance in M. tuberculosis
[DBT Govt. of India]

3. Efflux Protein Mediated Multidrug Resistance in coli
[UGC Major Research Projects]
  1. Pattanayak, B. S.; Priyadarshinee, M.; Dehury, B.; Jha, S.; Beuria, T. K.; Soren, D.; Mallick, B. C. Kanamycin mediated conformational dynamics of Escherichia coliouter membrane protein TolC. Frontiers in Molecular Biosciences 2021, 8, 636286. doi.org/10.3389/fmolb.2021.636286
  2. Badhai, S.; Barik,D.; Mallick, B.C. Anticancer efficacy of β-sitosterol loaded hydroxyapatite-alginate on colon cancer cell in-vivoResearch J. Pharm. and Tech.2020, 13(3), 1147-1151. doi:5958/0974-360x.2020.00211.5
  3. Mishra, S.; Priyadarshinee, M.; Debnath, A. K.; Muthe, K. P.; Mallick, C.; Das, N.; Parhi, P. Rapid microwave assisted hydrothermal synthesis cerium vanadate nanoparticle and its photocatalytic and antibacterial studies. Journal of Physics and Chemistry of Solids2020, 137, 10211-10219. doi.org/10.1016/j.jpcs.2019.109211
  4. Behera, N.; Arakha,M.; Soren, S.; Pattanayak, B. S.; Priyadashsinee, M.; Jha, M.; Mallick, C. Oxidative stress generated at nickel oxide nanoparticle interface results in bacterial membrane damage leading to cell death. RSC Advances20199, 24888-24894.  doi.org/10.1039/C9RA02082A
  5. Nayak, P. S.; Arakhaa, M.; Kumar,A.;Asthana,S.; Mallick, B.C.; Jha, S. Optimization of online silver nanoparticle biofabrication using Bacillus thuringiensisRSC Advances 2016, 6, 8232-8242. org/10.1039/C5RA21281B
  6. Arakhaa, M.; Pala, S.; Samantarraia,D.;Panigrahid, T.K.; Mallick, B. C.; Pramanikb, K.; Mallick, B.; Jha, S. Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interfaces. Scientific Reports 2015, 5:14813. doi:10.1038/srep14813.
  7. Mallick, B.C. Effects of Sarcosine on the Stability of Cytochrome C.IJSR 2014, 4;9, 2277-8179.
  8. Arakha, M.; Saleem, Md.; Mallick, B. C.; Jha, S. The effects of interfacial potential on antimicrobial propensity of ZnO nanoparticle, Scientific Reports 2015, 5: 9578. doi:10.1038/srep09578.
  9. Mallick, B.C* Kang, Sa-Ouk; Jha, S. Ca2+-binding induces conformational stability of CAF-1 from Dictyostelium discoideum Chem. Sci. 2014,126:3, 751–761.
  10. Mallick, B.C.; Kishore, N. Partial molar volumes of some alpha-amino acids in aqueous magnesium sulphate solutions at 298.15 K, J. Solution Chem. 2006, 35,1441-1451

Teaching :

1. Thermodynamics – Click to View PDF

2. Biophysical Chemistry

3. Error analysis

4. Bioinorganic Chemistry

5. Photochemistry